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Fig. 2 | Molecular Medicine

Fig. 2

From: Nitric Oxide Inhibits Endothelial IL-1β-induced ICAM-1 Gene Expression at the Transcriptional Level Decreasing Sp1 and AP-1 Activity

Fig. 2

NO released from S-nitrosocysteine (SNOC) inhibits IL-1β-dependent ICAM-1 mRNA expression in murine aortic endothelial cells in a concentration dependent manner.

Cells were incubated for 2 h without (lane 1) or with IL-1β (lanes 2–6) in the absence (lanes 1 + 2) or presence of SNOC (lanes 3–5) or with the denitrosated control compound SNOC−NO (lane 6). Subsequently, total cellular RNA was isolated and ICAM-1- and GAPDH-specific RT-PCR was performed. While IL-1β leads to a highly significant induction of ICAM-1 mRNA expression, SNOC significantly inhibits IL-1β-driven ICAM-1 transcription in a concentration dependent manner. Values are mean ± SD of four independent experiments.

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