Fig. 4
NO released from SNOC inhibits the DNA binding activities of transcription factors binding to the ZIP site, to both Sp1 sites, and to the AP-13 site of the murine ICAM-1 promoter. Murine aortic endothelial cells were incubated for 2 h without or with 200 Ulml IL-1β in the presence or absence of SNOC or the denitrosated control compound SNOC−NO. Subsequently, nuclear extracts were prepared. Gel shift experiments show that IL-1β induces increased binding of proteins to the ZIP site, two Sp1 sites, and the AP-13 site, and that SNOC in a concentration dependent manner decreases these DNA binding activities. Representative gel-shifts are shown.