Table 1 Oligonucleotide consensus sequences of transcription factor binding sites used for the gel-shift assays, and their position in the murine ICAM-1 5′ regulatory region relative to the translation start site (15,16)
Binding Site | Position | Sequence |
|---|---|---|
Sp11 | −73 | 5′-CTAGAAAAGCGCCGCCCGCCTCAT-3′ |
AP-11 | −94 | 5′-CTAGACCCCCGTGAGCCAGAGACT-3′ |
Sp12 | −112 | 5′-CTAGAACAGCACCGCCCCTCGGCT-3′ |
NF-κB1 | −142 | 5′-CTAGATTCCCGAGGTTTCCCGGAAAT-3′ |
AP-12 | −304 | 5′-CTAGATCTCCGGACTCACCTGCT-3′ |
NF-κB2 | −316 | 5′-CTAGAGGCCCGGGGCTTCTCTCCGGT-3′ |
AP-13 | −333 | 5′-CTAGAGAGGCGTGACTCCTGGAGGT-3′ |
AP-3 | −428 | 5′-CTAGAATCCCTGCGAAATGCCGAGCCT-3′ |
ZIP-site | −701 | 5′-CTAGAGGCCGCGGGGGCGGGAGCAGT-3′ |