Fig. 1
From: Human Endotoxemia Activates p38 MAP Kinase and p42/44 MAP Kinase, But Not c-Jun N-terminal Kinase
LPS activates p38 MAPK, p42/44 MAPK and JNK in PBMCs and non-PBMCs in vitro. PBMCs and non-PBMCs were isolated from whole blood, incubated for 45 min at 37°C, and stimulated with LPS for 15 min at the concentrations indicated. The leukocyte fraction (PBMC or non-PBMC) was loaded on one gel, in a quantity relative to their proportion in whole blood as to allow comparison of their relative contribution to MAPK activation in the total leukocyte fraction. MAPK activation was assessed on Western blot using phosphospecific antibodies for p38 MAPK (pp38, A), p42/44 MAPK (pp42/44, B), and JNK (pp46/pp54, C). Western blots shown are representative of three experiments.