Fig. 4
From: Human Endotoxemia Activates p38 MAP Kinase and p42/44 MAP Kinase, But Not c-Jun N-terminal Kinase
Human endotoxemia activates p38 MAPK. Peripheral blood leukocytes from LPS-treated volunteers were obtained just before LPS infusion (0′), and 5, 15, 30, and 60 min, and 4, 8 and 24 h after LPS infusion. p38 MAPK phosphorylation and enzymatic activity was then assessed at various time points. All Western blots shown are representative of at least three experiments. (A) p38 MAPK phosphorylation was determined using antibodies against phosphorylated p38 MAPK (pp38). (B) p38 MAPK enzymatic activity was determined by measuring phosphorylation of ATF-2 (pATF-2) in an in vitro kinase assay, using immunoprecipitated phosphorylated p38 MAPK from leukocyte lysates. (C) Kinetic analysis of p38 MAPK phosphorylation in leukocytes samples from 8 LPS-treated volunteers. Results (mean ± SEM) were obtained by image analysis of Western blotting with phosphospecific antibodies and expressed relative to a reference sample, which was set on a 100 arbitrary units.