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Fig. 5 | Molecular Medicine

Fig. 5

From: Human Endotoxemia Activates p38 MAP Kinase and p42/44 MAP Kinase, But Not c-Jun N-terminal Kinase

Fig. 5

Human endotoxemia activates p42/44 MAPK.

Peripheral blood leukocytes from LPS-treated volunteers were obtained just before LPS infusion (0′), and 5, 15, 30, and 60 min, and 4, 8 and 24 h after LPS infusion. P42/44 MAPK phosphorylation and enzymatic activity was then assessed at various time points. All Western blots shown are representative of at least three experiments. (A) p42/44 MAPK phosphorylation was determined using antibodies against phosphorylated p42/44 MAPK (pp42/44). (B) p42/44 MAPK enzymatic activity was determined by measuring phosphorylation of Elk-1 (pElk-1) in an in vitro kinase assay, using immunoprecipitated phosphorylated p42/44 MAPK from leukocyte lysates. (C) Kinetic analysis of p42/44 MAPK phosphorylation in leukocytes samples from LPS-treated volunteers (number of samples analyzed as indicate in the figure). Results (mean ± SEM) were obtained by image analysis of Western blotting with phosphospecific antibodies and expressed relative to a reference sample, which was set on a 100 arbitrary units.

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