Fig. 6
From: Human Endotoxemia Activates p38 MAP Kinase and p42/44 MAP Kinase, But Not c-Jun N-terminal Kinase
Human endotoxemia does not activate JNK. Peripheral blood leukocytes from LPS-treated volunteers were obtained just before LPS infusion (0′), and 5, 15, 30, and 60 min, and 4, 8 and 24 h after LPS infusion. JNK phosphorylation and enzymatic activity was then assessed at various time points. All Western blots shown are representative of at least three experiments. (A) JNK phosphorylation was determined using antibodies against phosphorylated JNK (pp46/pp54), UV light treated 293 cells served as a positive control for JNK phosphorylation (+). (B) JNK enzymatic activity was determined by measuring phosphorylation of c-Jun (p c-Jun) in an in vitro kinase assay, using immunoprecipitated phosphorylated JNK from leukocyte lysates, HeLa cells treated with osmoshock or left untreated served as a positive and negative control (+, −).