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Fig. 1 | Molecular Medicine

Fig. 1

From: Arsenite Activation of PI3K/AKT Cell Survival Pathway is Mediated by p38 in Cultured Human Keratinocytes

Fig. 1

Arsenite and H2O2 induce phosphorylation of p38, AKT and eNOS in cultured human keratinocytes. (a) Cultured human keratinocytes were treated with arsenite (200 µM) and H2O2, and cells were lysed at various time points. Protein extracts were subjected to SDS-PAGE and Westernblot analysis for phosphorylation of p38 or AKT or eNOS with anti-phospho-p38 or anti-phospho-AKT or anti-phospho-eNOS antibody respectively. Total p38, or AKT or eNOS was determined by stripping the membranes and re-probing with anti-p38, or anti-AKT, or anti-eNOS antibody. (b) Quantitative densitometric data for the phosphorylation p38, AKT and eNOS after arsenite treatment. The results from three independent experiments were analyzed and expressed as fold increase (mean ± SD, n = 3).

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Molecular Medicine

ISSN: 1528-3658