Fig. 2

Arsenite-induced activation AKT and eNOS is not mediated by antioxidant sensitive pathway but is mediated by PI3K and p38. Cultured human keratinocytes were pretreated with DPI (10 µM), or NAC (5 mM), or PDTC (10 µM), or LY (10 µM), WT (10 µM) for 2 hours, then treated with arsenite (200 µM) for 1 hour for analysis of p38 and AKT, and 2 hours for analysis of eNOS. Cells were lysed in RIPA buffer, and proteins were subjected to SDS-PAGE and Western blot analysis for phosphorylation of p38 or AKT with anti-phospho-p38 or anti-phospho-AKT or phospho-eNOS antibody, or for total p38 or AKT with anti-p38 or AKT or eNOS antibody.