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Fig. 3 | Molecular Medicine

Fig. 3

From: Arsenite Activation of PI3K/AKT Cell Survival Pathway is Mediated by p38 in Cultured Human Keratinocytes

Fig. 3

Arsenite induces ROS generation and inhibits phosphatase activity but does not activate EGFR. (A) Arsenite induces ROS generation. HaCat cells were loaded with 1 µM of DHR for 1 h, then treated with 200 µM of sodium arsenite, and cells were fixed at various time points post treatment. ROS production was measured by FACS analysis. Three independent experiments were performed. Presented here was the result of a representative experiment. (B) Arsenite inhibits phosphatase activity in cultured human keratinocytes. Cultured human keratinocytes were treated with arsenite (200 µM) and cells were collected at various time points. Phosphatase activity assay was performed as described in materials and methods. (C) Arsenite does not activate EGF receptor in cultured human keratinocytes. Cells were treated with EGF (100 ng/ml, as a positive control) or arsenite (200 µM) and cells were collected at various time points. Proteins from cell extracts were subjected to immunoprecipitation with anti-EGFR antibody and SDS-PAGE and Western blot analysis. Phosphorylated- or total-EGFR was probed with anti-PY-20 or anti-EGFR antibody.

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