Fig. 3From: Adenovirus-directed Expression of Dominant Negative Estrogen Receptor Induces Apoptosis in Breast Cancer Cells and Regression of Tumors in Nude MiceEffect of dominant negative ER on transcriptional activity of the endogenous ER in T47D cells. ER transcriptional activity was assayed using the estrogen-responsive luciferase reporter vector described in Materials and Methods. Triplicate wells of T47D cells were co-infected with 1 PFU/cell AdERE-Luc and increasing amounts (1, 5, and 10 PFU/cell) of AdGal, AdwtER, Ad1-536, or AdL540Q. T47D cells infected with AdERE-Luc alone were used as a control. After overnight infection, fresh medium containing E2 (1 nM) was added, cells were incubated for an additional 24 h, and luciferase activity was assayed. Data are expressed as a percentage (mean ± standard deviation, n = 3) of luciferase activity in control T47D cells treated with E2. Reporter activity in AdL540Q-infected cells is significantly (p < .01) reduced compared to Ad-Gal infected cells at MOIs of 5 and 10 PFU/cell; activity in Ad1-536-infected cells is significantly (p < .01) reduced compared to AdGal- and L540Q-infected cells at all MOIs. Similar results were obtained in two additional independent experiments.Back to article page