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Fig. 3 | Molecular Medicine

Fig. 3

From: Adenovirus-directed Expression of Dominant Negative Estrogen Receptor Induces Apoptosis in Breast Cancer Cells and Regression of Tumors in Nude Mice

Fig. 3

Effect of dominant negative ER on transcriptional activity of the endogenous ER in T47D cells. ER transcriptional activity was assayed using the estrogen-responsive luciferase reporter vector described in Materials and Methods. Triplicate wells of T47D cells were co-infected with 1 PFU/cell AdERE-Luc and increasing amounts (1, 5, and 10 PFU/cell) of AdGal, AdwtER, Ad1-536, or AdL540Q. T47D cells infected with AdERE-Luc alone were used as a control. After overnight infection, fresh medium containing E2 (1 nM) was added, cells were incubated for an additional 24 h, and luciferase activity was assayed. Data are expressed as a percentage (mean ± standard deviation, n = 3) of luciferase activity in control T47D cells treated with E2. Reporter activity in AdL540Q-infected cells is significantly (p < .01) reduced compared to Ad-Gal infected cells at MOIs of 5 and 10 PFU/cell; activity in Ad1-536-infected cells is significantly (p < .01) reduced compared to AdGal- and L540Q-infected cells at all MOIs. Similar results were obtained in two additional independent experiments.

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