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Fig. 1 | Molecular Medicine

Fig. 1

From: Developmental Expression of Wild-Type and Mutant Presenilin-1 in Hippocampal Neurons from Transgenic Mice: Evidence for Novel Species-Specific Properties of Human Presenilin-1

Fig. 1

Characterization of PS1 antibodies. Western blotting of mouse brain homogenates from control nontransgenic (tg−) and transgenic (tg+) animals expressing the wtPS1 human protein. Blots probed with the polyclonal antibody 17.2 raised to the PS1 C-terminal fragment (PS1-CTF; lanes 1, 2) demonstrated an ∼ 18 kD endoproteolytic fragment as well as a less intense PS1 holoprotein at ∼50 kD. The human-specific monoclonal NT1 (lanes 3, 4) detected a doublet of PS1 fragments only; little or no full-length human PS1 (PS1-FL) was observed in the transgenic brains. Molecular weight markers are as indicated. PS1-NTF, PS1 N-terminal fragment.

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Molecular Medicine

ISSN: 1528-3658