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Fig. 1 | Molecular Medicine

Fig. 1

From: Platelet Glycoprotein Ib: A Zinc-Dependent Binding Protein for the Heavy Chain of High-Molecular-Weight Kininogen

Fig. 1

SDS-PAGE analysis of proteins eluted from the LDC-27 affinity column. Fractions that reacted with LDC-27 were pooled, concentrated, and subjected to a 10% SDS-polyacrylamide gel (A) as well as ligand blot (B) analysis. In A, the gel was stained with Coomassie blue after electrophoresis. Lane 1 is the starting platelet membrane preparation, lane 2, the eluate from LDC-27 affinity column in the absence of zinc, and lane 3, the eluate from LDC-27 affinity column in the presence of zinc. (B) Ligand blot of the eluate from the affinity column in the presence of zinc using biotinylated HK (lane 1), and biotinylated LDC-27 (lane 2).

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Molecular Medicine

ISSN: 1528-3658