Fig. 2

Cerebral infarct volume is increased in HO2^−/− but not wild-type (WT) mice following MCAO. Mice were submitted to 1 hr MCAO and 23 hr reperfusion. (Top) Representative coronal brain sections from WT and HO2^−/− mice after MCA occlusion and reperfusion are shown. The infarct volume was determined by triphenyltetrazolium chloride staining. The white region representing the ischemic region was delineated and the surface area was measured. (Bottom) The infarct volume shown on the histogram was corrected for edema: the infarct represents 25 ± 3% of the hemisphere in WT (n = 9) and 44 ± 6% of the hemisphere in HO2^−/− (n = 7) mice (*p < 0.001). No WT mice died and one HO2^−/− mouse died.