Fig. 1
From: NO-Evoked Macrophage Apoptosis Is Attenuated by cAMP-Induced Gene Expression
Apoptotic parameters are unaffected by short-term lipophilic cAMP preincubation. RAW 264.7 macrophages were prestimulated for 1 hr with 1 mM 8-Br-cAMP or db-cAMP prior to the addition of NO donors. (A) DNA fragmentation in response to 1 mM GSNO was measured after 8 hr and quantitated with the diphenylamine assay. (B) p53 accumulation in response to 1 mM GSNO was determined after 4 hr by Western blot analysis. (C) Caspase-3-like cleavage of Ac-DEVD-AMC was analyzed after 4 hr following NO donor addition. Data are mean values ± SD of three individual experiments. For details and statistical analysis see Materials and Methods.