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Fig. 4 | Molecular Medicine

Fig. 4

From: NO-Evoked Macrophage Apoptosis Is Attenuated by cAMP-Induced Gene Expression

Fig. 4

Long-term lipophilic cAMP preincubation decreased apoptosis in human macrophages. Primary human monocyte-derived macrophages were prestimulated for 15 hr with 1 mM db-cAMP prior to stimulation with 1 mM GSNO, or remained as controls. (A) Annexin V staining was quantitated after 4 hr. Histograms show single-color labeling. (B) Expression of p53 in response to 1 mM GSNO was determined after 4 hr by Western blot analysis. (C) Caspase-3-like activity was measured by the cleavage of Ac-DEVD-AMC after 4 hr. (D) Mitochondrial membrane potential (Δψ) was assessed by DiOC6(3) measurements after 8 hr. Histograms show single-color labeling. Representative results are shown. For details and statistical evaluations see Materials and Methods.

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