Fig. 5.
From: Identification of two novel elements involved in human MUC1 gene expression in vivo
Detection of the −750 bp and −250 bp DNase I hypersensitive sites in MUC1 transgenic mice. Southern blots of DNase I digested chromatin from transgenic mouse (TG) or wild type (WT) tissue samples digested with BamH I and hybridised with human specific (A, C, E) and mouse specific (B, D, F) MUC1/muc1 probes. For each panel, Lane 1 shows DNA prepared from nuclei which had not been treated with DNase I. Lanes 2–5 show DNA prepared from nuclei treated with a constant amount of DNase I (40U) for increasing lengths of time, 2, 2 min; 3, 5 min; 4, 10 min; 5, 15 minutes. The human-specific probe detects 3 genomic fragments and DHS at 6.5 and 6 kb in chromatin from stomach (A) lung (C) and kidney (E). The mouse specific-probe detects a genomic fragment of about 12 kb and DHS in stomach only (B) at 5.6, 5.0 and 4.6 kb but not in lung (D) or kidney (F).