Treated by | Relative Count (%) |
---|
NANase III | 0.0 |
NDV sialidase | 85.0 |
N-glycanase | 96.3 |
Nontreated fetuin | 100.0 |
- [14C] sialylated fetuin was prepared as described in Materials and Methods. Subsequent to sialidase and N-glycanase treatment, each sample was subjected to SDS-PAGE. The radioactive materials in fetuin were visualized with a BAS2000 radio image analyzer and the incorporated radioactivity was counted.