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Table 2 Linkage analysis of incorporated sialic acids

From: Identification and Functional Characterization of a Human GalNAc α2,6-Sialyltransferase with Altered Expression in Breast Cancer

Treated by

Relative Count (%)

NANase III

0.0

NDV sialidase

85.0

N-glycanase

96.3

Nontreated fetuin

100.0

  1. [14C] sialylated fetuin was prepared as described in Materials and Methods. Subsequent to sialidase and N-glycanase treatment, each sample was subjected to SDS-PAGE. The radioactive materials in fetuin were visualized with a BAS2000 radio image analyzer and the incorporated radioactivity was counted.