Table 1 Primers used to amplify LYST cDNA fragments
From: The Chediak-Higashi Protein Interacts with SNARE Complex and Signal Transduction Proteins
LYST Bait, (bp*) | Primer Sequence, 5′ →3′ (forward, upper sequence; reverse, lower sequence) | Restriction Enzyme | Bait Size (bp) |
|---|---|---|---|
190–1056 | GCGGTGGATCCCATGAGCACCGACAGTAACTCAC GCGGAATTCTCATAGTGTGGGCACTACACTGG | BamHI EcoRI | 866 |
1051–1950 stathmin-like | GCGGTGGATCCCACACTAACTGAGTTCCTAGCAGGCTTTGGGGACTGC (GAGAGA)3CTCGAGTCAATCCATACAACAGCATATTCCAATG | BamHI Xho1 | 899 |
1948–2370 | GCGGAACTAGTGGATCCCAAATCTGTAATCATTCC GGAATTCTCACTGAACAACTATATTGCCTTTCTG | SpeI EcoRI | 422 |
2347–3213 | GCGAGTGTGGATCCCCAGAAAGGCAATATAGTTGTTCAG GCGGAATTCTCACTCCTCTTTGTGACTTCTGAAC | BamHI EcoRI | 866 |
3190–4032 | GCGGTGGATCCCCTGTTCAGAAGTCACAAAGAGG GGAATTCTCAAGCAGAAAGCAAATTTAATTCCAG | BamHI EcoRI | 842 |
4009–4821 | GCGGTGGATCCCCTGGAATTAAATTTGCTTTCTGC GCGGAATTCTCAGGAGCCCAGTGAAATTATATG | BamHI EcoRI | 812 |
4819–5700 | GCGGTGGATCCCTCCAAAGCGTTGATGATCCAAG GCGGAATTCTCAAACTCGCAGTGCTAATGCTTG | BamHI EcoRI | 881 |
5677–6612 | GCGGTGGATCCCACTCAAGCATTAGCACTGCGAG (GAGAGA)3CTCGAGTCAGGCAACATAAGTATCTGCAATATTTTG | BamHI Xhol | 935 |
6586–7449 | GCGGTGGATCCCCAAAATATTGCAGATACTTATGTTGCC GCGGAATTCTCATCCCATGTTTCTCACATCTTCCAG | BamHI EcoRI | 863 |
7426–8238 | GCGGTGGATCCCCTGGAAGATGTGAGAAACATGGG GCGGAATTCTCAGGTCTGGAAAACTGAGGTCTTG | BamHI EcoRI | 812 |
8218–9039 | GCGGTGGATCCCAAGACCTCAGTTTTCCAGACCG GCGGAATTCTCAATCCAACTGCCATGAGGTTGG | BamHI EcoRI | 821 |
9037–9585 | GCGGTGGATCCCGATCCAACAGAAGGGCCAAATC GCGGAATTCTCATGTCAGAGCGGTGATGTTACC | BamHI EcoRI | 548 |
9502–10590 BEACH dom. | GCGGTGGATCCCAAGGTTCGTGATGATGTATACCAC GCGGAATTCTCACAAGCCTTTTATCCATGACAAAGG | BamHI EcoRI | 1088 |
10576–11611 WD-40 dom. | GCGGTGGATCCCTGGATAAAAGGCTTGAAATGGGG GCGGAATTCTCATGAAGTTCATTCGCATTCACCC | BamHI EcoRI | 1035 |