Table 1 Primer sequences derived from rat cDNAs used in the RT-PCR assays
Primers | Sequences | Amplification size | Annealing temperature/cycles (n) |
|---|---|---|---|
TF Upstream 107 Downstream 230 | 5′-CCACCTTTCTCGGCTTCCTTC-3′ 5′-GGTTTCGGTTGCCACTCCAAG-3′ | 126 bp | 61°C/29 |
TFPI Upstream 272 Downstream 600 | 5′-TGCAAAGCAATGATACGGAG-3′ 5′-CTGCACTCCTCCAAGGTCTC-3′ | 329 bp | 60°C/32 |
t-PA Upstream 704 Downstream 1088 | 5′-CACAGCTTTACCACATCCAA-3′ 5′-CAGATAGCACCCAGCAGGAA-3′ | 385 bp | 60°C/28 |
PAI-1 Upstream 851 Downstream 1170 | 5′-GACATCCTGGAACTGCCCTA-3′ 5′-ACCTCGATCTTGACCTTTTG-3′ | 320 bp | 60°C/28 |
GAPDH Upstream 34 Downstream 333 | 5′-GTGAAGGTCGGTGTCAACG-3′ 5′-GGTGAAGACGCCAGTAGACTC-3′ | 300 bp | 55°C/27 |