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Table 1 Primer sequences derived from rat cDNAs used in the RT-PCR assays

From: Hemostasis Imbalance in Experimental Hypertension

Primers Sequences Amplification size Annealing temperature/cycles (n)
TF
Upstream 107 Downstream 230
5′-CCACCTTTCTCGGCTTCCTTC-3′ 5′-GGTTTCGGTTGCCACTCCAAG-3′ 126 bp 61°C/29
TFPI
Upstream 272 Downstream 600
5′-TGCAAAGCAATGATACGGAG-3′ 5′-CTGCACTCCTCCAAGGTCTC-3′ 329 bp 60°C/32
t-PA
Upstream 704 Downstream 1088
5′-CACAGCTTTACCACATCCAA-3′ 5′-CAGATAGCACCCAGCAGGAA-3′ 385 bp 60°C/28
PAI-1
Upstream 851 Downstream 1170
5′-GACATCCTGGAACTGCCCTA-3′ 5′-ACCTCGATCTTGACCTTTTG-3′ 320 bp 60°C/28
GAPDH
Upstream 34 Downstream 333
5′-GTGAAGGTCGGTGTCAACG-3′ 5′-GGTGAAGACGCCAGTAGACTC-3′ 300 bp 55°C/27