Fig. 2

Immunofluorescence in semithin cryosections of HSV-1 infected and gD-1 (A, C, D, F) or gC-1 (B, E) labeled human fibroblasts showing the effects of BFA (C) and the reversibility of BFA influence (D, E, F). The plasma membranes (arrows) were unlabeled and the glycoproteins piled up in a gigantic perinuclear space (arrowhead) in some BFA-treated cells (C). In the cytoplasm, scattered spots of glycoproteins were presented in unperturbed and BFA-treated cells up to 15 min following release, after which the glycoproteins were transported widespread to the cytoplasm in a reticular pattern (D). Nearly all glycoproteins accumulated in Golgi-like areas after removal of BFA for 3 hr (E, arrowhead), although some labeled adhesion areas could be identified. When BFA was washed away for 9 hr, the labeling of the plasma membranes (arrows) were nearly restored (F). Bars: A–F = 10 µm.