Fig. 6

Ultrathin cryosections of HSV-1-infected fibroblasts treated with BFA (A, B) and after removal of BFA for 30 min (C) and 6 hr (D). Both gD-1 (A and C) and gC-1 (B) were demonstrable in the nuclear membranes (arrowheads). The capsids accumulated at reduplicated nuclear membranes (arrows C, D). After 6 hr washout, the nuclei were nearly exhausted of capsids (D). Tubulin was marked with GAR-G15 (A) or GAR-G5 (D). Bars: A–C = 0.15 µm; D = 0.4 µm.