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Fig. 3 | Molecular Medicine

Fig. 3

From: PI3K Blockade by Ad-PTEN Inhibits Invasion and Induces Apoptosis in Radial Growth Phase and Metastatic Melanoma Cells

Fig. 3

Melanoma cells undergo apoptosis following Ad-PTEN, but not Ad-Luc, treatment. (A) WM35, A375-S2, and MeWo cells were treated with 5000 vp/cell viruses, harvested 4 days after infection, the Annexin V assay performed, and the cells analyzed by flow cytometry. The peak signal of apoptotic cells are shown with arrows. The percentage of apoptotic cells is indicated. (B) Ad-PTEN regulates cell-cycle progression in melanoma cells. WM35 and MeWo cells were treated with 2500 vp/cell of Ad-PTEN or Ad-Luc and 3 days postinfection; cells were harvested and stained with PI for cell-cycle analysis using a FACScan. Ad-PTEN treatment causes a G2/M block in tumor cells as indicated by arrows.

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Molecular Medicine

ISSN: 1528-3658