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Fig. 3 | Molecular Medicine

Fig. 3

From: Lack of Plasma Membrane Targeting of a G172D Mutant Thiamine Transporter Derived from Rogers Syndrome Family

Fig. 3

Multiple amino acid substitutions of the THTR-1 at position 172. NIH3T3 cells were transfected with pCan expression plasmids encoding for THTR-1 mutants as indicated by the three-letter code amino acid substitutions at residue 172. Proteins from cell lysates were resolved on a 10% SDS-PAGE followed by Western blotting with anti-myc 9E11 monoclonal antibody and antimouse IgG conjugated to HRP (Santa Cruz Inc.) followed by chemiluminescence detection.

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Molecular Medicine

ISSN: 1528-3658