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Fig. 6 | Molecular Medicine

Fig. 6

From: Lack of Plasma Membrane Targeting of a G172D Mutant Thiamine Transporter Derived from Rogers Syndrome Family

Fig. 6

Cellular colocalization of glycosylated and unglycosylated THTR-1 protein with β-cop protein. Swiss 3T3 cells were cotransfected with GFP expression plasmid, together with THTR-1 wild-type expression plasmid, and either left untreated or treated with tunicamycin (10 µg/ml) for 24 hr prior to cell fixation. Cells were stained with anti-myc 9E11 monoclonal antibody and anti-β-cop rabbit polyclonal antibody. The following panels are shown: GFP stain (green, panel A), myc-THTR-1 stain using CY3 conjugated goat anti-mouse (red, panel B), β-cop stain using CY5 conjugated goat anti-rabbit (blue, panel C). The white arrow at the top panel in C indicates the staining of the Golgi compartment. The convergence of all three filters is shown in panel D. The white arrows at the bottom panel in D indicate the staining of the Golgi (left) and ER (right) compartments.

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