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Fig. 7 | Molecular Medicine

Fig. 7

From: Lack of Plasma Membrane Targeting of a G172D Mutant Thiamine Transporter Derived from Rogers Syndrome Family

Fig. 7

[3H]-Thiamine uptake in Swiss 3T3 cells transfectants expressing the wild-type and mutant G172D THTR-1 proteins. [3H]-Thiamine uptake was measured in Swiss 3T3 cells ectopically expressing the indicated proteins. Net thiamine uptake was calculated by subtraction of the radioactivity obtained at 37°C with that observed at 4°C. Net thiamine uptake in parental Swiss 3T3 cells was determined as 100%, whereas wild-type and G172D mutant THTR-1 expressing cells were calculated relatively. The 100% thiamine uptake of Swiss 3T3 cells corresponds to an average of 192 pmols/106 cells/20 min. Results shown represent the average and standard error of the mean derived from three independent experiments. The cells were incubated with [3H]-thiamine for 20 min in a transport buffer at pH 7.9 as described in materials and methods.

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