Fig. 4

Flow cytometry analysis of P. anomala MUCL 41969 cells stained with PI. Yeast cells (10⁶) were incubated for 1 hr in the presence of 300 µg/ml of purified WmKT in 25 mM of sodium acetate buffer (pH 4.6) (A), 300 µg/ml of purified WmKT pretreated with 25 µM castanospermine (B), buffer alone (C), or buffer containing 25 µM castanospermine (D). After staining, cell-associated fluorescence was determined.