Fig. 5From: Involvement of β-Glucans in the Wide-Spectrum Antimicrobial Activity of Williopsis saturnus var. mrakii MUCL 41968 Killer ToxinGlucosidase activity of WmKT. Purified WmKT (1.8 µg)in 50 µl 25 mM of sodium acetate (pH 4.6) was incubated for 10 hr at 26°C in the presence of 1 mM of p-nitrophenyl β-D-glucopyranoside. The incubation was performed in the absence (A) or in the presence (B) of 25 µM of castanospermine. The control experiment was performed using boiled WmKT (C). p-Nitrophenol release was monitored and expressed as micromoles of p-nitrophenol per microgram of WmKT. Means and standard deviations were calculated from three independent experiments.Back to article page