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Fig. 5 | Molecular Medicine

Fig. 5

From: Involvement of β-Glucans in the Wide-Spectrum Antimicrobial Activity of Williopsis saturnus var. mrakii MUCL 41968 Killer Toxin

Fig. 5

Glucosidase activity of WmKT. Purified WmKT (1.8 µg)in 50 µl 25 mM of sodium acetate (pH 4.6) was incubated for 10 hr at 26°C in the presence of 1 mM of p-nitrophenyl β-D-glucopyranoside. The incubation was performed in the absence (A) or in the presence (B) of 25 µM of castanospermine. The control experiment was performed using boiled WmKT (C). p-Nitrophenol release was monitored and expressed as micromoles of p-nitrophenol per microgram of WmKT. Means and standard deviations were calculated from three independent experiments.

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