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Fig. 2 | Molecular Medicine

Fig. 2

From: Overexpression of the Shb SH2 Domain-Protein in Insulin-Producing Cells Leads to Altered Signaling Through the IRS-1 and IRS-2 Proteins

Fig. 2

Proliferation (A) and apoptosis (B) of GFP-RIN and GFP + Shb-RIN cells. 2000 GFP-RIN or GFP + Shb-RIN cells were plated in culture dishes on day 0 (day of cell sorting and one day after lipofection). On days 1, 2, and 3 cells were stained for 10 min with 10 µg/ml of PI and detached by trypsination. Cells were then counted (proliferation) and analyzed for FL3 fluorescence (apoptosis) by flow cytometry in a Becton-Dickinson FACSCalibur. Results are means for three independent observations. *p < 0.05 and **p < 0.01 versus corresponding control using Student’s paired t-test.

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Molecular Medicine

ISSN: 1528-3658