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Fig. 5 | Molecular Medicine

Fig. 5

From: Overexpression of the Shb SH2 Domain-Protein in Insulin-Producing Cells Leads to Altered Signaling Through the IRS-1 and IRS-2 Proteins

Fig. 5

Interactions of RINm5F cell proteins with Shb GST fusion proteins. Immobilized GST Shb SH2 and GST Shb PTB domain fusion proteins (10 µg each) were mixed with cell lysates of nonstimulated or insulin-stimulated RINm5F cells. In one group, 20 mM phosphotyrosine was included in the incubation mixture. After 30 min, the beads were washed and subjected to immunoblot analysis for phosphotyrosine (4G10) and FAK.

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