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Fig. 6 | Molecular Medicine

Fig. 6

From: Overexpression of the Shb SH2 Domain-Protein in Insulin-Producing Cells Leads to Altered Signaling Through the IRS-1 and IRS-2 Proteins

Fig. 6

Effects of FCS and insulin on PI3K activity in RIN-Neo and RIN-Shb cells. RIN-Neo and RIN-Shb cells were serum deprived for 60 min followed by stimulation with 10% FCS or 10 µg/ml insulin. Phosphotyrosine proteins were immunoprecipitated from homogenates and analyzed for PI3K activity. PI3K activity was related to the total homogenate protein content and expressed as fold-increase as compared with serum-deprived RIN-Neo cells. Results are means ± SEM for three to eight observations. The data were analyzed for statistical significance using a one-way ANOVA on repeated measures (p < 0.05). Individual comparisons were performed using a Dunnet’s test when comparing with the corresponding untreated controls.

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Molecular Medicine

ISSN: 1528-3658