Fig. 1
From: GTK Tyrosine Kinase-induced Alteration of IRS-protein Signalling in Insulin Producing Cells
Insulin-induced IRS-1 and IRS-2 phophorylation in islet cells. 2000 islets from control (n = 9) and GTK-transgenic (n = 6) mice were isolated and divided equally into two groups (1000 islets/group). The islets were serum starved for 60 min and stimulated with insulin dissolved in 30 mM HCl (+) or HCl only (−) for 10 min or left unstimulated. The cells were lysed and subjected to immunoprecipitation for IRS-1 followed by immunoprecipitation for IRS-2. All precipitates were subjected to Western blot analysis for phosphotyrosine (P-Tyr). Cell lysate was subjected to SDS-PAGE and Western blot analysis for ERK1/2 (A). Densitometric scanning was performed and the IRS-phosphorylation normalised against the amount of lysate ERK1/2 or immunoprecipitated total IRS-2 was calculated for each group (B and C) in two separate experiments. Means ± SEM are given.