Skip to main content
Fig. 4 | Molecular Medicine

Fig. 4

From: GTK Tyrosine Kinase-induced Alteration of IRS-protein Signalling in Insulin Producing Cells

Fig. 4

Insulin-induced PI3K activity in RINm5F cells. (A) Cells were stimulated with insulin as described above, immunoprecipitated with phosphotyrosine antibody (PY20) and subjected to an in vitro kinase reaction using PI as a substrate for PI3K. Phospholipids were then separated on silica TLC plates and the radioactive product (PIP) was detected with autoradiography (A) and quantified by densitometric scanning (B). The stimulation of PI3K activity as percent of maximum was calculated as described above (C). Bars are means ± SEM from 4 independent experiments. *, p < 0.05 and **, p < 0.01 when compared with corresponding value of RINm5F-control (neo-1 and neo-2).

Back to article page