Fig. 6
From: GTK Tyrosine Kinase-induced Alteration of IRS-protein Signalling in Insulin Producing Cells
Insulin-induced SHB-phosphorylation in RINm5F cells. (A) Serum-starved control (neo) RINm5F cells and RINm5F cells overexpressing GTK were stimulated with insulin as described above. The cells were then washed, lysed and immunoprecipitated for SHB and subjected to Western blot analysis for phosphotyrosine (P-Tyr), SHB, IRS-2 and phospho-FAK. Densitometric scanning of phosphorylated SHB was performed and the mean normalised against the total amount of SHB was calculated from 4 independent experiments. Bars are means ± SEM. * p < 0.05 when compared with corresponding value of RINm5F-control (neo-1). (B) Cells (neo-1 and GTKY504F) cultured in a 10% serum medium were immunoprecipitated for SHB and subjected to Western blotting for phosphotyrosine (P-Tyr) and SHB.