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Fig. 1 | Molecular Medicine

Fig. 1

From: Selective Inhibition of FLICE-like Inhibitory Protein (FLIP) Expression With Small Interfering RNA Oligonucleotides (siRNAs) Is Sufficient to Sensitize Tumor Cells for TRAIL-Induced Apoptosis

Fig. 1

FLIP-specific short double-stranded RNA oligonucleotides inhibit FLIP expression. (A and B) KB cells stably transfected with fusion proteins of FLIP-L (A) and FLIP-S (B) carboxy-terminally tagged with EGFP (KB-FLIP-L-GFP; KB-FLIP-S-GFP) were transfected twice on 2 successive days by electroporation with the indicated siRNAs. One day after the second round of electroporation expression of the GFP, chimeras were analyzed by FACS (solid line) and compared with parental KB cells (grey), untreated KB-FLIP-L-GFP (dotted lines in A) and KB-FLIP-S-GFP cells (dotted lines in B). (C and D) KB-FLIP-L-GFP (C), KB-FLIP-S-GFP (C), KB (D), and SV80 cells (D) were electroporated twice with the indicated siRNAs as described and after 1 day of recovery total RNAs were isolated. RPA analyses were performed to detect transcripts of the indicated genes using the Multi-Probe template set hApo-3b (PharMingen). Protected transcripts were separated by denaturing polyacrylamide gel electrophoresis (5%) and visualized on a PhosphorImager. Protected FLIP transcripts were normalized with respect to RNA loads using the L32 internal expression control.

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