Selective Inhibition of FLICE-like Inhibitory Protein (FLIP) Expression With Small Interfering RNA Oligonucleotides (siRNAs) Is Sufficient to Sensitize Tumor Cells for TRAIL-Induced Apoptosis

Table 2 siRNA-mediated reduction of FLIP-S-GFP expression in KB-FLIP-S-GFP cells

siRNA	hr	Positive Cells (%)	Mean Intensity	Relative Expression	Reduction (%)
—	24	46	30	1380	0
siRNA-F1	24	9	20	180	87
siRNA-F2	24	15	28	420	70
F1+F2	24	16	27	432	69
siRNA-neo	24	48	30	1440	−4
—	48	53	37	1961	0
siRNA-F1	48	29	22	638	68
siRNA-F2	48	23	27	621	68
F1+F2	48	27	27	729	63
siRNA-neo	48	54	37	1998	−2

Twenty-four and 48 hr, after two rounds of electroporation with the indicated siRNAs (150 nM), cells were analyzed by FACS to determine relative FLIP-S-GFP expression. The product of percentage of positive cells and the corresponding mean fluorescence intensity of mock electroporated cells was defined as 100%.

ISSN: 1528-3658