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Fig. 6 | Molecular Medicine

Fig. 6

From: Adenoviral-Induced Islet Cell Cytotoxicity Is Not Counteracted by Bcl-2 Overexpression

Fig. 6

Effects of adenoviral exposure on islet cell viability. Cells were transduced with different concentrations of adenoviral vector AdCMVProg for 60 min at 37 °C and cell viability was assessed 7 days postinfection. Cells were cultured in the absence of RU 486. (a) XTT assay. Individual colorimetric readings are normalized to the value of nontransduced, nontreated cells. Results are mean ± SD of eight separate experiments. (b) Micrographs showing bisbenzimide/propidium iodide fluorescence of control and adenovirus-exposed (30 PFU/cell) cells. Nuclear blue bisbenzimide staining reflects viable cells, whereas red propidium/iodide staining reflects necrotic cells. (c) Electron microscopy studies on control and adenovirus exposed (30 PFU/cell) cells. β-Cell necrosis is characterized by vacuolization and the loss of both plasma membrane and nuclear membrane integrity.

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