Fig. 1

Proteolysis of radiolabeled amidated bovine complement C3 by an activity found in preparations of the E. granulosus HCW. ¹²⁵I-C3(NH₃) (11 ng/track) was incubated (1 min at 37°C, 50 µl total volume) with or without 30 µl of the 2 M NaCl extract from HCW. Reaction mixtures were added to SDS-PAGE sample buffer, further incubated for 20 min at 37°C, run on SDS-PAGE (7.5% w/v acrylamide, reducing conditions) and autoradiographed. The 44-kDa (highly labeled) and 80-kDa (slightly labeled) fragments from the α chain of C3(NH₃) generated by the proteolytic activity under study are indicated.