Fig. 2From: Expression of the Proteinase Specialized in Bone Resorption, Cathepsin K, in Granulomatous InflammationPurification of the proteolytic activity. The PBS extract of the HCW was fractionated on resource Q anion exchange (A) and the active fractions (bar) rechromatographed on superose 12 gel filtration (B). Activity was followed on the basis of the cleavage of 125I-C3(NH3) by SDS-PAGE and autoradiography. Active fractions in (B) are indicated by asterisks.Back to article page