Fig. 1From: Acute Intermittent Porphyria: Expression of Mutant and Wild-Type Porphobilinogen Deaminase in COS-1 CellsWestern blot analysis of the wild-type and mutated polypeptides. To visualize the PBGD polypeptides expressed by the wild-type and mutated constructs, cell lysates were run on a SDS polyacrylamide gel, transferred onto a nitrocellulose filter, and immunostained using a polyclonal rabbit PBGD antibody.Back to article page