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Fig. 3 | Molecular Medicine

Fig. 3

From: Acute Intermittent Porphyria: Expression of Mutant and Wild-Type Porphobilinogen Deaminase in COS-1 Cells

Fig. 3

Pulse-chase expression analysis of the wild-type and mutant PBGD polypeptides in COS-1 cells. During 1-hr pulse, all synthesized polypeptides were labeled with 35S-cysteine and 35S-methionine, and thereafter synthesized proteins were chased for 1, 4, and 23 hr, respectively. The wild-type and mutant polypeptides were immunoprecipitated and visualized by autoradiography after running the samples on an SDS-PAGE.

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Molecular Medicine

ISSN: 1528-3658