Table 3 PBGD activity assay after expression in COS-1 cells
Construct | Enzyme activity (±S.E.) nmol/mg prot/ha | Percentage of wild type activityb |
|---|---|---|
SVpoly-PBGD | 3173 (±507) | 100% |
SVpoly-PBGD-R26C | 105 (±17) | 0% |
SVpoly-PBGD-R167W | 273 (±66) | 5% |
SVpoly-PBGD-R173W | 173 (±33) | 1% |
SVpoly-PBGD-R173Q | 140 (±11) | 0% |
SVpoly-PBGD-R225G | 626 (±78) | 16% |
SVpoly-PBGD-R225X | 142 (±9) | 0% |
SVpoly-PBGD + 43 aa (1073delA) | 1660 (±174) | 50% |
SVpoly | 131 (±3) | 0% |
H2O | 113 (±13) | 0% |
COS-1 cells, not transfected | 127 (±7) | 0% |