Fig. 1

Changes in plasma insulin, glucose and PAI-1 levels after insulin treatment of obese (ob/ob) and lean mice. ob/ob and lean mice were injected intraperitoneally with saline or 10 units of regular human insulin. The animals were sacrificed at the indicated times and the blood was collected into 20 mM EDTA (final concentration). After cen-trifugation, the plasma was collected and plasma insulin (panel A), glucose (panel B), and PAI-1 (panel C) levels were determined as indicated in Materials and Methods. Each time point on the graph represents the mean ±SD of six animals. ◼: lean, 12–14 weeks; ▲: ob/ob, 12–14 weeks; ▼: ob/ob, 24–28 weeks