Skip to main content
Fig. 3 | Molecular Medicine

Fig. 3

From: Insulin Continues to Induce Plasminogen Activator Inhibitor 1 Gene Expression in Insulin-Resistant Mice and Adipocytes

Fig. 3

Effects of insulin on glucose transport and PAI-1 mRNA expression in normal and insulin-resistant 3T3-L1 adipocytes. Fully differentiated 3T3-L1 adipocytes were treated for 3 days with buffer (normal adipocytes; ◼) or with 3 ng/ml recombinant murine TNF-± to render them insulin resistant (◊). (A) The effect of insulin on 2-deoxy-d-[1,2-3H] glucose uptake was determined as described in Materials and Methods. (B) The effect of insulin on PAI-1 mRNA expression was determined 3 hr after exposure to insulin using quantitative RT-PCR. For panels A and B, n = 6 ± SD.

Back to article page

Molecular Medicine

ISSN: 1528-3658