Fig. 3
Reduction in the amount of cellular Ras in SW480 cells by FTS. 5 × 105 cells were diluted directly in media containing either 100 µM of FTS or control solvent and plated in 10-cm dishes. After 4 days of treatment, with fresh media and drug added every 24 hr, cells were harvested and analyzed for Ras content by Western blotting. Actin served as an internal control to demonstrate equal protein amounts in the respective lanes. Densitometric analysis of three separate experiments showed a 2-fold reduction of total cellular Ras in FTS-treated cells; one of three representative experiments is shown.