Fig. 5
Regulation of Ras and p53 in HT-29 cells. 5 × 105 cells were diluted directly in media containing either 100 µM of FTS or control solvent and plated in 10-cm dishes. After 4 days of treatment, with fresh media and drug added every 24 hr, cells were harvested and analyzed for Ras and p53 content by Western blotting. Actin served as an internal control to demonstrate equal protein amounts in the respective lanes. Densitometric analysis showed a 1.5-fold reduction of total cellular Ras and a 3-fold induction of p53 expression in FTS-treated cells. A representative experiment is shown.