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Fig. 2 | Molecular Medicine

Fig. 2

From: Suppression of Type I Collagen Gene Expression by Prostaglandins in Fibroblasts Is Mediated at the Transcriptional Level

Fig. 2

Regulation of the − 976 bp Col1a1 promoter-CAT construct by hIL-1α and indomethacin in BALBc/3T3 fibroblasts. BALBc/3T3 fibroblasts were transfected with the Col1a1-CAT construct containing the sequence spanning −976/+116 bp. The cells were then treated with increasing concentrations of hIL-1α, as indicated, in the absence (solid bars) or presence (cross-hatched bars) of 10 −6 M indomethacin and CAT activity was determined after 48 hr by the fluor diffusion method. Values are means ± SEM of triplicate plates.

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