Fig. 3From: Suppression of Type I Collagen Gene Expression by Prostaglandins in Fibroblasts Is Mediated at the Transcriptional LevelRegulation of Col1a1 promoter-CAT constructs by hIL-1 α, indomethacin, and PGE2.BALBc/3T3 fibroblasts were transfected with CAT constructs containing the sequences spanning − 976/+116 bp (lanes l–5) or −222/+116 bp (lanes 6-10) of the Col1a1 promoter. CAT activity was determined after 48 hr of treatment without (lanes l and 6) or with 10−6 M indomethacin (lanes 2 and 7), 10 pM hIL-1α, (lanes 3 and 8), IL-1 plus indomethacin (lanes 4 and 9), or IL-1, indomethacin, and 250 nM PGE2 (lanes 5 and 10). The values are means ± SEM from two experiments with duplicate plates. The scheme represents positions of DNA elements defined previously (33,40,67).Back to article page