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Fig. 6 | Molecular Medicine

Fig. 6

From: Suppression of Type I Collagen Gene Expression by Prostaglandins in Fibroblasts Is Mediated at the Transcriptional Level

Fig. 6

EMSA analysis of protein-DNA complexes in nuclear extracts from prostaglandin-treated BALBc/3T3 fibroblasts. The double-stranded oligonucleotide probe (Coll/56) encompassing the sequence spanning −84 to −29 bp of the Col1a1 promoter was end-labeled with 32P and incubated alone (first lane showing free probe only) or with 5 µg of nuclear extract from untreated (control) or PGE1-, PGE2-, or PGF2α-treated BALBc/3T3 cells. The protein-DNA complexes A-E were separated on a 4% polyacrylamide gel. The complexes A, B, and E were competed by excess unlabeled probe titrated at 10×, 50×, 100×, and 200 ×, and complexes C and D were nonspecific (not shown).

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