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Fig. 3 | Molecular Medicine

Fig. 3

From: Lack of Interferon-γ Production Despite the Presence of Interleukin-18 During Cutaneous Wound Healing

Fig. 3

IFN-γ is not expressed at the wound site. (A) Frozen serial sections from 7-day mouse wounds were isolated from Balb/c animals. Sections were incubated with a polyclonal antiserum directed against murine IFN-γ. All sections were stained with the avidin-biotin-peroxidase complex system using 3-amino-9-ethylcarbazole as a chromogenic substrate. Nuclei were counterstained with hematoxylin. Scale bar = 50 µm. IFN-γ-immunopositive signals within the sections could not be detected (he, hyperproliferative epithelium; g, granulation tissue). (B) A frozen serial section from the spleen of an endotoxin (LPS)-treated mouse was used as a positive control for the validity of the anti-IFN-γ anti-serum. Strongly IFN-γ-immunopositive cells were indicated by arrows. Scale bar = 25 µm.

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